Journal: Reproductive Sciences

Article Title: Mechanisms of LncRNA FTX in Regulating Islet Function of Pregnant Mice Born With Low-Protein Diet-Induced Intrauterine Growth Retardation

doi: 10.1007/s43032-025-01870-2

Figure Lengend Snippet: LncRNA FTX was insufficiently down-regulated in IUGR pregnant mice. The ( A ) heatmap of differentially expressed lncRNAs between IUGR pregnant mice and normal pregnant mice. The ( B ) KEGG enrichment and ( C ) GO analysis on target mRNA of differentially expressed LncRNA. ( D ) The localization of lncRNA FTX in chromosome X. ( E ) Expression levels of lncRNA FTX in different tissues of 8-week-old normal control mice, compared with islets. ( F ) LncRNA FTX expression level in islets of NC, NC pregnancy, IUGR, IUGR pregnancy, compared with non-pregnancy. ( G ) The expression level of LncRNA FTX in TC6 cells under different glucose concentrations, compared with 25 mM. NC, Normal Control mice; NP, Normal Control pregnant mice; IUGR, Intrauterine Growth Restriction; IP, Intrauterine Growth Restriction pregnant mice. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: In order to investigate the effect of lncRNA FTX on mouse islet cell line β-TC6, we ordered three interference sequences of lncRNA FTX from Shanghai GenePharma Co., LTD.

Techniques: Expressing, Control

Journal: Reproductive Sciences

Article Title: Mechanisms of LncRNA FTX in Regulating Islet Function of Pregnant Mice Born With Low-Protein Diet-Induced Intrauterine Growth Retardation

doi: 10.1007/s43032-025-01870-2

Figure Lengend Snippet: Down-regulation of LncRNA FTX promoted cell proliferation and the expression of genes related to insulin synthesis and secretion, and inhibited cell apoptosis. ( A ) Interference efficiency of three LncRNA FTX interfering sequences in TC6 cells, compared with the control group. ( B ) The representative graph of apoptosis ratio of TC6 cells after downregulation of lncRNA FTX, compared with the mock group. ( C ) Proliferation activity of TC6 cells after FTX intervention 24 h and 48 h later, compared with the mock group. ( D ) Expression of apoptosis-related proteins in TC6 cells after downregulation of lncRNA FTX, compared with the mock group. The expression of insulin-related genes after lncRNA FTX was down-regulated in TC6 cells at ( E ) nucleic acid level, compared with the mock group. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: In order to investigate the effect of lncRNA FTX on mouse islet cell line β-TC6, we ordered three interference sequences of lncRNA FTX from Shanghai GenePharma Co., LTD.

Techniques: Expressing, Control, Activity Assay

Journal: Reproductive Sciences

Article Title: Mechanisms of LncRNA FTX in Regulating Islet Function of Pregnant Mice Born With Low-Protein Diet-Induced Intrauterine Growth Retardation

doi: 10.1007/s43032-025-01870-2

Figure Lengend Snippet: Overexpression of lncRNA FTX inhibited cell proliferation and the expression of insulin-related genes, and promoted apoptosis. ( A ) The overexpression efficiency of pcDNA-FTX in TC6 cells, compared with the empty-vector group. ( B ) The representative graph of apoptosis ratio of TC6 cells after the overexpression of lncRNA FTX, compared with the empty-vecor group. ( C ) The proliferation activity of TC6 cells after FTX was over-expressed, compared with the empty-vecor group. ( D ) The expression of apoptosis-related protein after the overexpression of lncRNA FTX in TC6 cells, compared with the empty-vecor group. The expression of insulin-related genes at ( E ) nucleic acid level after the overexpression of FTX in TC6 cells, compared with the empty- vector group. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: In order to investigate the effect of lncRNA FTX on mouse islet cell line β-TC6, we ordered three interference sequences of lncRNA FTX from Shanghai GenePharma Co., LTD.

Techniques: Over Expression, Expressing, Plasmid Preparation, Activity Assay

Journal: Reproductive Sciences

Article Title: Mechanisms of LncRNA FTX in Regulating Islet Function of Pregnant Mice Born With Low-Protein Diet-Induced Intrauterine Growth Retardation

doi: 10.1007/s43032-025-01870-2

Figure Lengend Snippet: FTX was a sponge of miR- 22 - 3p which regulated pten through PI3 K/AKT signaling pathway. ( A ) StarBase V2.0 predicted that miR- 22 - 3p was a target of lncRNA FTX and pten was a target of miR- 22 - 3p. The levels of miR- 22 − 3p ( B ) and pten ( C ) in NC, NC pregnancy, IUGR, IUGR pregnancy, compared with non-pregnancy. ( D ) Dual luciferase report assay in WT- lncRNA FTX or MUT-lncRNA FTX co-transfected with miR-NC or miR- 22 - 3p mimics. ( E ) Dual luciferase assay in WT-pten or MUT-pten co-transfected with miR-NC or miR- 22 - 3p mimics, compared with NC mimics.The mRNA expression level of ( F ) pten and the protein expression levels of ( G ) p-PI3 K, PI3 K, p-AKT and AKT after down-regulation of lncRNA FTX in TC6 cells, compared with the mock group. The mRNA expression level of ( H ) pten and the protein expression levels of ( I ) p-PI3 K, PI3 K, p-AKT and AKT after the overexpression of lncRNA FTX in TC6 cells, compared with the empty-vector group. NC, Normal Control mice; IUGR, Intrauterine Growth Restriction.* p < 0.05, ** p < 0.01, * p < 0.001

Article Snippet: In order to investigate the effect of lncRNA FTX on mouse islet cell line β-TC6, we ordered three interference sequences of lncRNA FTX from Shanghai GenePharma Co., LTD.

Techniques: Luciferase, Transfection, Expressing, Over Expression, Plasmid Preparation, Control